烟草原生质体生产酚类化合物。

Phenolics production by encapsulated Nicotiana tabacum cells.

机构信息

Department of Agricultural and Chemical Engineering, Colorado State University, 80523, Fort Collins, CO, USA.

出版信息

Plant Cell Rep. 1989 Dec;8(8):475-8. doi: 10.1007/BF00269052.

Abstract

Plant cells of tobacco (Nicotiana tabacum L.) were grown for several generations in suspension cultures. Cells were immobilized in continuous bioreactors in calcium alginate (Ca Alg) beads or in poly-L-lysine (PLL) encapsulated calcium alginatehydrogels. In each case, the cells were fed continuously a modified Linsmaier-Skoog plant cell culture medium. The bioreactor effluent was analyzed for total phenolic compounds. The net specific productivity of phenolics was calculated on a daily basis for several test runs. For comparison, productivity in suspension cultures was monitored. Productivity of suspended cells declined to zero within 9 d; both immobilized and encapsulated cells remained productive for 16 d following inoculation. Specific productivity by encapsulated cells was higher than that by immobilized cells; in both types similar rates of decline in productivity occurred.

摘要

烟草（Nicotiana tabacum L.）细胞在悬浮培养中经过几代生长。细胞在连续生物反应器中通过海藻酸钙（CaAlg）珠或聚-L-赖氨酸（PLL）包封的海藻酸钙水凝胶固定化。在每种情况下，细胞都连续喂食改良的林塞默-斯科格植物细胞培养液。分析生物反应器流出物中的总酚类化合物。根据几个测试运行，以每日为基础计算酚类物质的净比生产率。为了进行比较，监测了悬浮培养中的生产力。悬浮细胞的生产力在 9 天内下降到零；接种后，固定化和包封细胞都保持了 16 天的生产力。包封细胞的比生产率高于固定化细胞；在这两种类型中，生产率下降的速度相似。