Marini P, Vitadello M, Bianchi R, Triban C, Gorio A
Diabetologia. 1986 Apr;29(4):254-8. doi: 10.1007/BF00454886.
The anterograde and retrograde axonal flow of acetylcholinesterase were studied in the sciatic nerve of alloxan-diabetic rats after five weeks of experimental diabetes. A slight reduction of the anterograde axonal flow of the enzyme was found in alloxan-diabetic compared to control rats. Sedimentation analysis revealed a major reduction of anterograde axonal flow of the light globular forms of the enzyme (G1 + G2), which are probably conveyed by slow transport. There was also a minor reduction of the anterograde flow of the globular form G4, while no modification of the axonal flow of the heavy asymmetric form A12 was found. Both G4 and A12 molecular forms are conveyed by fast axonal transport. In contrast, no abnormality of the retrograde axonal flow of acetylcholinesterase was observed. Ganglioside treatment antagonized the decline of the anterograde axonal flow of the enzyme in alloxan-diabetic rats. These results are consistent with the view that experimental diabetic neuropathy is associated with axonal transport defects, and suggest a protective effect of ganglioside treatment against neuronal damage(s) related to the diabetic syndrome.
在实验性糖尿病五周后，对四氧嘧啶糖尿病大鼠的坐骨神经中乙酰胆碱酯酶的顺行和逆行轴突运输进行了研究。与对照大鼠相比，发现四氧嘧啶糖尿病大鼠中该酶的顺行轴突运输略有减少。沉降分析显示，该酶的轻球状形式（G1 + G2）的顺行轴突运输大幅减少，这些轻球状形式可能由慢速运输携带。球状形式G4的顺行运输也略有减少，而重不对称形式A12的轴突运输未发现改变。G4和A12分子形式均由快速轴突运输携带。相反，未观察到乙酰胆碱酯酶逆行轴突运输的异常。神经节苷脂治疗拮抗了四氧嘧啶糖尿病大鼠中该酶顺行轴突运输的下降。这些结果与实验性糖尿病神经病变与轴突运输缺陷相关的观点一致，并提示神经节苷脂治疗对与糖尿病综合征相关的神经元损伤具有保护作用。
