Shipp M A, Ahmed P, Kannapell C C, Ford J C, McCourt D, Leykam J F, Zacheis M, Bono C, Davie J M, Mustain E
Hum Immunol. 1986 May;16(1):24-37. doi: 10.1016/0198-8859(86)90033-9.
In man, the immune response genes are located within the HLA-D/DR region, and the gene products, the Ia antigens, are expressed on B lymphocytes, monocytes, and a percentage of null cells and activated T lymphocytes. We recently identified a human Ia antigen, K19, which appeared to be limited in its expression to B lymphocytes, and to be preferentially expressed on the more mature cells within this population. This work was facilitated by a monoclonal antibody. HK-19, which recognized a monomorphic determinant of this Ia molecule. We now report the characterization of a second monoclonal antibody, HK-13, which recognized the same molecule as HK-19, but only on cells from some individuals. The greater affinity of HK-13 allowed more complete characterization of the K19/K13 molecule. This characterization included cytofluorography, two-dimensional gel electrophoresis, tryptic peptide mapping, and partial N-terminal amino acid sequencing, and indicated that K19 and K13 were epitopes on HLA-DQ (DC) molecules. The pattern of reactivity of HK-13 on a panel of typing cells did not correlate with any of the known HLA-DQ polymorphic determinants. Thus, HK-13 is a new polymorphic determinant of the HLA-DQ series.
在人类中，免疫反应基因位于HLA - D/DR区域内，其基因产物即Ia抗原，在B淋巴细胞、单核细胞以及一定比例的裸细胞和活化T淋巴细胞上表达。我们最近鉴定出一种人类Ia抗原K19，其表达似乎仅限于B淋巴细胞，并且在该群体中更成熟的细胞上优先表达。这项工作借助一种单克隆抗体HK - 19得以推进，该抗体识别这种Ia分子的一个单态决定簇。我们现在报告第二种单克隆抗体HK - 13的特性，它识别与HK - 19相同的分子，但仅在某些个体的细胞上识别。HK - 13更高的亲和力使得对K19/K13分子能够进行更全面的特性分析。这种特性分析包括细胞荧光术、二维凝胶电泳、胰蛋白酶肽图谱分析以及部分N端氨基酸测序，结果表明K19和K13是HLA - DQ（DC）分子上的表位。HK - 13在一组分型细胞上的反应模式与任何已知的HLA - DQ多态决定簇均不相关。因此，HK - 13是HLA - DQ系列的一个新的多态决定簇。
