Ababou A, Goyeneche J, Davis W C, Lévy D
URA-INRA, Immuno Pathologie Cellulaire et Moléculaire, Ecole Nationale Vétérinaire, Alfort, France.
J Leukoc Biol. 1994 Aug;56(2):182-6. doi: 10.1002/jlb.56.2.182.
Studies were conducted to determine the reactivity of six monoclonal antibodies specific for major histocompatibility complex class II molecules expressed on a bovine B cell line homozygous for BoLA-DR and BoLA-DQ alleles. Direct immunoprecipitation, serial immunodepletion experiments, and two-dimensional gel electrophoresis revealed that these antibodies reacted with three different molecules. Bovine orthologues of HLA-DR were recognized by three monoclonal antibodies--H34A, TH12A, and TH14B. Orthologues of HLA-DQ were characterized by two other monoclonal antibodies, TH22A and TH81A. A third BoLA class II antigen, neither DR nor DQ was revealed by the last monoclonal antibody H42A. The relation of this molecule to known molecules in humans and other species remains to be established. However, cumulative data suggest that the determinant is expressed on a molecule related to HLA-DP.
开展了多项研究，以确定六种单克隆抗体的反应性，这些抗体针对在BoLA - DR和BoLA - DQ等位基因纯合的牛B细胞系上表达的主要组织相容性复合体II类分子具有特异性。直接免疫沉淀、连续免疫去除实验和二维凝胶电泳表明，这些抗体与三种不同的分子发生反应。三种单克隆抗体——H34A、TH12A和TH14B识别出了HLA - DR的牛直系同源物。另外两种单克隆抗体TH22A和TH81A鉴定出了HLA - DQ的直系同源物。最后一种单克隆抗体H42A揭示了第三种BoLA II类抗原，既不是DR也不是DQ。该分子与人类及其他物种中已知分子的关系仍有待确定。然而，累积数据表明，该决定簇在与HLA - DP相关的分子上表达。
