Bontrop R, van Miltenburg R, Koning F, Gerrets R, Hyland C, Giphart M
Hum Immunol. 1986 May;16(1):38-51. doi: 10.1016/0198-8859(86)90034-0.
Class II molecules were isolated from consanguineous HTCs (DR1-DRw8) by sequential immunoprecipitation with the monoclonal antibodies 7.3.19.1 (anti-DRw52-like), B8.11.2 (anti-DR backbone), and 7.5.10.1 (anti-HLA class II backbone). Depending upon the DR-serotype of the cell line used, two or three class II antigen families, distinct in molecular weight, could be isolated (see Hum Immunol 9:221, 1984). Immunoprecipitated class II molecules were treated with NaNase and then analyzed on 1D-IEF gels. Each HLA class II antigen family contained two alpha chains conserved in pI. Furthermore, the various haplotypes show distinct electrophoretic beta chain patterns. The number of beta chain charge configurations detected varies from 2 to 5, depending upon the antigen family or haplotype studied. Some of these chains have a pI which is specific for a given class II serotype whereas other beta chain pIs are invariant and shared among more antigen families or haplotypes.
通过用单克隆抗体7.3.19.1（抗DRw52样）、B8.11.2（抗DR主干）和7.5.10.1（抗HLA II类主干）进行连续免疫沉淀，从近亲HTC（DR1-DRw8）中分离出II类分子。根据所用细胞系的DR血清型，可以分离出两个或三个分子量不同的II类抗原家族（见《人类免疫学》9:221，1984）。将免疫沉淀的II类分子用核酸酶处理，然后在一维IEF凝胶上进行分析。每个HLA II类抗原家族包含两条在等电点上保守的α链。此外，各种单倍型显示出不同的电泳β链模式。检测到的β链电荷构型数量从2到5不等，这取决于所研究的抗原家族或单倍型。其中一些链具有特定于给定II类血清型的等电点，而其他β链等电点是不变的，并且在更多抗原家族或单倍型之间共享。
