Bontrop R, Tilanus M, Mikulski M, van Eggermond M, Termijtelen A, Giphart M
Immunogenetics. 1986;23(6):401-5. doi: 10.1007/BF00372674.
HLA-DR molecules were isolated from eight different HLA-DR3 homozygous B-cell lines by immunoprecipitation with monoclonal antibodies, and they were subsequently analyzed by two-dimensional gel electrophoresis. We found that HLA-DR3 homozygous B-cell lines of consanguineous origin express two types of HLA-DR molecules. One type of HLA-DR molecule was present in all the cell lines tested, whereas the second DR molecule appears to be polymorphic. DNA isolated from the different HLA-DR3 homozygous cell lines was studied by Southern blot analysis to determine whether any DR beta restriction fragment length polymorphism could be observed. Polymorphisms detected at both the product and genomic level have been compared to each other, and their relations to the serological (HLA-DR) and cellular (HLA-D and LB-Q1) typing data will be discussed.
通过用单克隆抗体进行免疫沉淀，从八个不同的HLA - DR3纯合B细胞系中分离出HLA - DR分子，随后通过二维凝胶电泳对其进行分析。我们发现，有血缘关系的HLA - DR3纯合B细胞系表达两种类型的HLA - DR分子。一种类型的HLA - DR分子存在于所有测试的细胞系中，而第二种DR分子似乎具有多态性。通过Southern印迹分析研究了从不同的HLA - DR3纯合细胞系中分离的DNA，以确定是否可以观察到任何DRβ限制性片段长度多态性。已将在产物水平和基因组水平检测到的多态性相互比较，并将讨论它们与血清学（HLA - DR）和细胞分型（HLA - D和LB - Q1）数据的关系。
