IEEE Trans Biomed Eng. 2014 Mar;61(3):705-10. doi: 10.1109/TBME.2013.2287218. Epub 2013 Oct 24.
This paper describes the synthesis of a 9-mers-long peptide ladder structure of glycine on a modified glass surface using a nanoliter droplet ejector. To synthesize peptide on a glass substrate, SPOT peptide synthesis protocol was followed with a nozzleless acoustic droplet ejector being used to eject about 300 droplets of preactivated amino acid solution to dispense 60 nL of the solution per mer. The coupling efficiency of each mer was measured with FITC fluorescent tag to be 96%, resulting in net 70% efficiency for the whole 9-mer-long peptide of glycine. Usage of a nanoliter droplet ejector for SPOT peptide synthesis increases the density of protein array on a chip.
本文描述了一种在改良的玻璃表面上使用纳升液滴喷射器合成甘氨酸 9 肽梯结构的方法。为了在玻璃基底上合成肽，采用 SPOT 肽合成方案，使用无喷嘴声滴喷射器将约 300 个预激活氨基酸溶液液滴喷射到每个 mer 中分配 60nL 的溶液。使用 FITC 荧光标记物测量每个 mer 的偶联效率为 96%，导致整个甘氨酸 9 肽的净效率为 70%。纳升液滴喷射器用于 SPOT 肽合成可增加芯片上蛋白质阵列的密度。
