Ajioka M, Nagai S, Ogawa K, Satake T, Sugiyama S, Ozawa T
J Electrocardiol. 1986 Apr;19(2):165-72. doi: 10.1016/s0022-0736(86)80024-3.
To clarify the mechanism of reperfusion arrhythmia, the following experiments were performed. In vivo study: Using anesthetized mongrel dogs, the left anterior descending coronary artery was occluded for 15 min and the ligation was released. The dogs were divided into two groups depending on whether the pretreatment was with saline or coenzyme Q10 (CoQ10), 15 mg/kg, before the ligation, i.e., the control and the CoQ10 groups. Each group was further divided into two subgroups depending on the presence or the absence of reperfusion arrhythmia. Reperfusion arrhythmia was observed in 12 out of 38 dogs in the control, whereas in the CoQ10 group none developed arrhythmia. Nine species of free fatty acids (FFA) were detected in the plasma membrane in each group. In the dogs in the control group with arrhythmia, all species of detected FFA increased, and phospholipid content in plasma membrane decreased. These changes were not observed in the dogs without arrhythmia in both the control and the CoQ10 groups. In vitro study: Incubation of myocardial plasma membrane with phospholipase (PLase) A2 increased only unsaturated FFA, while PLase C increased all detected FFA. Premedication with CoQ10 prevented the increase in FFA caused by PLases. Perfusion with PLase A2 or C altered membrane action potential. Premedication with CoQ10 also prevented changes in membrane action potential. PLase liberates fatty acids from phospholipids, and CoQ10 is known to protect the membrane phospholipids from the attack of PLase. These facts and results suggest that activation of PLase associated with coronary reperfusion is closely related to the development of reperfusion arrhythmia.
为阐明再灌注性心律失常的机制，进行了以下实验。体内研究：使用麻醉的杂种犬，将左冠状动脉前降支闭塞15分钟后松开结扎。根据结扎前预处理是使用生理盐水还是15mg/kg辅酶Q10（CoQ10），将犬分为两组，即对照组和CoQ10组。每组根据是否发生再灌注性心律失常进一步分为两个亚组。对照组38只犬中有12只观察到再灌注性心律失常，而CoQ10组无一只发生心律失常。每组的质膜中检测到9种游离脂肪酸（FFA）。对照组中发生心律失常的犬，所有检测到的FFA种类均增加，质膜中的磷脂含量降低。对照组和CoQ10组中未发生心律失常的犬未观察到这些变化。体外研究：心肌质膜与磷脂酶（PLase）A2孵育仅增加不饱和FFA，而PLase C增加所有检测到的FFA。CoQ10预处理可防止PLases引起的FFA增加。用PLase A2或C灌注可改变膜动作电位。CoQ10预处理也可防止膜动作电位的变化。PLase从磷脂中释放脂肪酸，已知CoQ10可保护膜磷脂免受PLase的攻击。这些事实和结果表明，与冠状动脉再灌注相关的PLase激活与再灌注性心律失常的发生密切相关。
