Akopian H R, Huleiuk N L, Kushniruk V O, Mykytenko D O, Iatsyshyna A P, Lukash L L
Tsitol Genet. 2013 Sep-Oct;47(5):55-69.
Long-term cultivation of human cells, including stem cells, can lead to substantial transformation of the karyotype and occurrence of genetic instability. The aim of this research was a comparative cytogenetic study of the karyotype of a new human stem cell line 4BL at 160 and 205 passages. The absence of 10 and 13 pairs of chromosomes and the monosomy of chromosomes 4, 8, 10, 11, 13, 15, 17, 21, X were observed; also six regular marker chromosomes were detected. Chromosomes 1, 15 and 21 are involved in translocations t(l;11), t(5;15), t(12; 15), t(16;21). Modal class of the karyotype is within 41-43 chromosomes at both 160 and 205 passages. The frequency of polyploid cells have been increased from 2.8% at 160 passage up to 36% at 205 passage. Cells with a near-haploid karyotype were not detected at 205 passage (in contrast to 24.6% at 160 passages) and a decline of the level of premature separation of chromatids was observed. We assume stabilization of karyotype of the cell line 4BL at 205 passage and consider that further research is needed to predict the direction of karyotypic evolution of these cells in vitro.
长期培养人类细胞，包括干细胞，会导致核型的显著改变和遗传不稳定性的出现。本研究的目的是对新的人类干细胞系4BL在第160代和第205代时的核型进行比较细胞遗传学研究。观察到第10和13对染色体缺失，以及染色体4、8、10、11、13、15、17、21、X单体；还检测到6条规则的标记染色体。染色体1、15和21参与了t(1;11)、t(5;15)、t(12;15)、t(16;21)易位。在第160代和第205代时，核型的众数类在41 - 43条染色体范围内。多倍体细胞的频率从第160代时的2.8%增加到第205代时的36%。在第205代未检测到近单倍体核型的细胞（与第160代时的24.6%相比），并且观察到染色单体过早分离水平下降。我们推测4BL细胞系在第205代时核型稳定，并认为需要进一步研究来预测这些细胞在体外核型进化的方向。
