Meisner L F, Wu S Q, Christian B J, Reznikoff C A
State Laboratory of Hygiene, University of Wisconsin Health Sciences Center, Madison 53706.
Cancer Res. 1988 Jun 1;48(11):3215-20.
Cytogenetic analysis at the 15th, 34th, 50th, and 56th passages of an SV40 immortalized human uroepithelial cell line (SV-HUC-1) showed continuous chromosome change and marker formation. Throughout these passages the transformed cells maintained their epithelial morphology, were SV40 T antigen positive, did not shed infectious SV40 virus, and were repeatedly found to be nontumorigenic when innoculated into athymic nude mice. Each of the passages studied was characterized by extensive karyotypic changes due to formation, rearrangement, and disappearance of different markers. A marker involving chromosome 1 was stable at three of the passages studied, whereas markers involving the X chromosome changed at each passage studied. Because of the incorporation of several chromosomes or chromosome arms into markers, the karyotype was genetically balanced in the first passage studied, with no net loss or gain of chromosomal material despite a modal number of 44. In subsequent passages, despite continued instability and generation of new markers, there was a slight but additive loss of genomic balance which increased with time in culture. Since continued karyotypic rearrangements did not lead to tumorigenic conversion, it is probable that genetic instability coupled with selection for the most balanced genome may be important for the immortalization of this cell line.
对一株SV40永生化人尿道上皮细胞系（SV - HUC - 1）在第15、34、50和56代进行的细胞遗传学分析显示，染色体持续变化并形成标记物。在这些传代过程中，转化细胞保持其上皮形态，SV40 T抗原呈阳性，不释放具有感染性的SV40病毒，并且当接种到无胸腺裸鼠体内时，多次被发现无致瘤性。所研究的每一代都具有因不同标记物的形成、重排和消失而导致的广泛核型变化特征。一个涉及1号染色体的标记物在所研究的三代中保持稳定，而涉及X染色体的标记物在每一代所研究的过程中都发生变化。由于几条染色体或染色体臂并入标记物中，在所研究的第一代中核型在遗传上是平衡的，尽管众数为44，但染色体物质没有净损失或增加。在随后的传代中，尽管持续不稳定并产生新的标记物，但基因组平衡有轻微但累加的损失，且随着培养时间的延长而增加。由于持续的核型重排并未导致致瘤性转化，遗传不稳定与对最平衡基因组的选择相结合可能对该细胞系的永生化很重要。
