Jimpy小鼠：髓鞘相关糖蛋白及其他蛋白质的定量分析

Jimpy mice: quantitation of myelin-associated glycoprotein and other proteins.

作者信息

Yanagisawa K, Quarles R H

出版信息

J Neurochem. 1986 Jul;47(1):322-5. doi: 10.1111/j.1471-4159.1986.tb02866.x.

DOI:10.1111/j.1471-4159.1986.tb02866.x

Abstract

Myelin-associated glycoprotein (MAG), 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) activity, myelin basic protein (BP), and proteolipid protein (PLP) were quantitated in the brains of 20-day-old Jimpy and control mice. The levels of MAG, CNPase, and BP in Jimpy brains were 5.3%, 9.7%, and 1.9% of those in control brains, respectively. Immunoblotting analysis did not reveal an increased apparent Mr for MAG in the Jimpy mouse, as has been observed in some other hypomyelinating murine mutants. PLP was reduced more than the other proteins, as it was not detected by an immunoblotting technique that was capable of detecting 0.5% of the control level.

摘要

对20日龄的Jimpy小鼠和对照小鼠的脑内髓鞘相关糖蛋白（MAG）、2',3'-环核苷酸3'-磷酸二酯酶（CNPase）活性、髓鞘碱性蛋白（BP）和蛋白脂蛋白（PLP）进行了定量分析。Jimpy小鼠脑内MAG、CNPase和BP的水平分别为对照小鼠脑内相应水平的5.3%、9.7%和1.9%。免疫印迹分析未发现Jimpy小鼠的MAG表观分子量增加，而在其他一些髓鞘形成低下的小鼠突变体中曾观察到这种现象。PLP的减少程度比其他蛋白质更甚，因为采用能够检测到对照水平0.5%的免疫印迹技术未检测到PLP。