Yang Fan, Liu Zhicheng, Dong Weifeng, Zhu Linghuan, Chen Xiaoyi, Li Xianzhen
School of Biological Engineering, Dalian Polytechnic University, Ganjingqu, Dalian, People's Republic of China.
Liaoning Entry-Exit Inspection and Quarantine Bureau, Zhongshanqu, Dalian, People's Republic of China.
Biotechnol Appl Biochem. 2014 Jul-Aug;61(4):418-25. doi: 10.1002/bab.1181. Epub 2014 Mar 13.
An inulin-degrading strain L610, which was competent to directly convert inulin into ethanol, was isolated and identified as a strain of Saccharomyces cerevisiae according to physiological and phylogenetic analysis. Ion chromatography results showed that isolate L610 could assimilate the intact inulin completely without acidic or enzymatic pretreatment in contrast to the previously reported strains of S. cerevisiae, which could only ferment the fructo-oligosaccharides with a degree of polymerization less than 15. Strain L610 yielded 37.2 g/L ethanol within 48 H at a shake flask level under the evaluated culture conditions (11% inulin, 0.4% yeast extract, and 0.05% MgSO4 at 30 °C and pH 6.0). The conversion efficiency of inulin-type sugar to ethanol was 60% of the theoretical ethanol yield. Strain L610 produced 40.0 g/L of ethanol when directly fermented in Jerusalem artichoke (Helianthus tuberosus L.) powder suspension within 24 H, which was higher than the reported data, 28.9 g/L, produced by S. cerevisiae KCCM 50549.
分离得到一株菊粉降解菌株L610，该菌株能够直接将菊粉转化为乙醇，根据生理和系统发育分析，鉴定为酿酒酵母菌株。离子色谱结果表明，与先前报道的只能发酵聚合度小于15的低聚果糖的酿酒酵母菌株相比，分离株L610无需酸性或酶预处理就能完全同化完整的菊粉。在评估的培养条件下（30℃、pH 6.0，含11%菊粉、0.4%酵母提取物和0.05%硫酸镁），菌株L610在摇瓶水平下48小时内产生37.2 g/L乙醇。菊粉型糖转化为乙醇的效率为理论乙醇产量的60%。当在菊芋（Helianthus tuberosus L.）粉末悬浮液中直接发酵24小时时，菌株L610产生40.0 g/L乙醇，高于酿酒酵母KCCM 50549报道的数据28.9 g/L。
