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利用未经预处理进行菊粉水解的酿酒酵母 KCCM50549 从菊芋粉中进行乙醇发酵。

Ethanol fermentation from Jerusalem artichoke powder using Saccharomyces cerevisiae KCCM50549 without pretreatment for inulin hydrolysis.

机构信息

Industrial Biotechnology Research Center, Daejeon, Republic of Korea.

出版信息

Bioresour Technol. 2011 Jan;102(2):2109-11. doi: 10.1016/j.biortech.2010.08.044. Epub 2010 Aug 24.

DOI:10.1016/j.biortech.2010.08.044
PMID:20833540
Abstract

A strain of Saccharomyces cerevisiae, KCCM50549, was found to efficiently ferment the inulin-containing carbohydrates in Jerusalem artichoke without acidic or enzymatic pretreatment prior to fermentation. S. cerevisiae KCCM50549 could utilize almost completely the fructo-oligosaccharides present in Jerusalem artichoke (up to degree of polymerization (DP) of 15), in contrast to the other S. cerevisiae strain such as NCYC625 that fermented the fructo-oligosaccharides with DP of up to around six. Inulin-fermenting S. cerevisiae KCCM50549 produced c.a. 1.6 times more ethanol from Jerusalem artichoke compared with S. cerevisiae NCYC625. Direct ethanol fermentation of Jerusalem artichoke flour at 180 g/L without any supplements or pretreatments by S. cerevisiae KCCM50549 in a 5 L jar fermentor yielded 36.2 g/L of ethanol within 36 h. The conversion efficiency of inulin-type sugars to ethanol was 70% of the theoretical ethanol yield.

摘要

一株酿酒酵母(Saccharomyces cerevisiae),KCCM50549,在发酵前无需进行酸性或酶预处理,即可有效地发酵菊苣中含有的碳水化合物。与其他酿酒酵母菌株(如 NCYC625)相比,S. cerevisiae KCCM50549 可以几乎完全利用菊苣中存在的低聚果糖(聚合度高达 15),而 NCYC625 则只能发酵聚合度在 6 左右的低聚果糖。与 S. cerevisiae NCYC625 相比,菊苣发酵酿酒酵母 KCCM50549 从菊苣中产生的乙醇量约多 1.6 倍。KCCM50549 在 5 L 罐式发酵罐中直接发酵 180 g/L 的菊苣粉,无需任何补充物或预处理,36 h 内可产生 36.2 g/L 的乙醇。菊糖型糖转化为乙醇的转化率为理论乙醇产量的 70%。

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