Industrial Biotechnology Research Center, Daejeon, Republic of Korea.
Bioresour Technol. 2011 Jan;102(2):2109-11. doi: 10.1016/j.biortech.2010.08.044. Epub 2010 Aug 24.
A strain of Saccharomyces cerevisiae, KCCM50549, was found to efficiently ferment the inulin-containing carbohydrates in Jerusalem artichoke without acidic or enzymatic pretreatment prior to fermentation. S. cerevisiae KCCM50549 could utilize almost completely the fructo-oligosaccharides present in Jerusalem artichoke (up to degree of polymerization (DP) of 15), in contrast to the other S. cerevisiae strain such as NCYC625 that fermented the fructo-oligosaccharides with DP of up to around six. Inulin-fermenting S. cerevisiae KCCM50549 produced c.a. 1.6 times more ethanol from Jerusalem artichoke compared with S. cerevisiae NCYC625. Direct ethanol fermentation of Jerusalem artichoke flour at 180 g/L without any supplements or pretreatments by S. cerevisiae KCCM50549 in a 5 L jar fermentor yielded 36.2 g/L of ethanol within 36 h. The conversion efficiency of inulin-type sugars to ethanol was 70% of the theoretical ethanol yield.
一株酿酒酵母(Saccharomyces cerevisiae),KCCM50549,在发酵前无需进行酸性或酶预处理,即可有效地发酵菊苣中含有的碳水化合物。与其他酿酒酵母菌株(如 NCYC625)相比,S. cerevisiae KCCM50549 可以几乎完全利用菊苣中存在的低聚果糖(聚合度高达 15),而 NCYC625 则只能发酵聚合度在 6 左右的低聚果糖。与 S. cerevisiae NCYC625 相比,菊苣发酵酿酒酵母 KCCM50549 从菊苣中产生的乙醇量约多 1.6 倍。KCCM50549 在 5 L 罐式发酵罐中直接发酵 180 g/L 的菊苣粉,无需任何补充物或预处理,36 h 内可产生 36.2 g/L 的乙醇。菊糖型糖转化为乙醇的转化率为理论乙醇产量的 70%。