Jin Han Jun, Kim Ki Cheol, Yoon Cha Eun, Kim Wook
Department of Nanobiomedical Science, Dankook University, Cheonan 330-714, Republic of Korea.
J Forensic Leg Med. 2013 Nov;20(8):1093-7. doi: 10.1016/j.jflm.2013.09.027. Epub 2013 Oct 14.
We analyzed the variation of eighteen miniSTR loci in 411 randomly chosen individuals from Korea to increase the probability that a degraded sample can be typed, as well as to provide an expanded and reliable population database. Six multiplex PCR systems were developed (multiplex I: D1S1677, D2S441 and D4S2364; multiplex II: D10S1248, D14S1434 and D22S1045; multiplex III: D12S391, D16S3253 and D20S161; multiplex IV: D3S4529, D8S1115 and D18S853; multiplex V: D6S1017, D11S4463 and D17S1301; multiplex VI: D5S2500, D9S1122 and D21S1437). Allele frequencies and forensic parameters were calculated to evaluate the suitability and robustness of these non-CODIS miniSTR systems. No significant deviation from Hardy-Weinberg equilibrium expectations were observed, except for D4S2364, D5S2500 and D20S161 loci. A multidimensional scaling plot based on allele frequencies of the six miniSTR loci (D1S1677, D2S441, D4S2364, D10S1248, D14S1434 and D22S1045) showed that Koreans appeared to have most genetic affinity with Chinese and Japanese than to other Eurasian populations compared here. The combined probability of match calculated from the 18 miniSTR loci was 2.902 × 10(-17), indicating a high degree of polymorphism. Thus, the 18 miniSTR loci can be suitable for recovering useful information for analyzing degraded forensic casework samples and for adding supplementary genetic information for a variety of analyses involving closely related individuals where there is a need for additional genetic information.
我们分析了从韩国随机选取的411名个体中18个微型短串联重复序列（miniSTR）位点的变异情况，以提高降解样本分型的概率，并提供一个扩展且可靠的群体数据库。我们开发了6个多重聚合酶链反应（PCR）系统（多重体系I：D1S1677、D2S441和D4S2364；多重体系II：D10S1248、D14S1434和D22S1045；多重体系III：D12S391、D16S3253和D20S161；多重体系IV：D3S4529、D8S1115和D18S853；多重体系V：D6S1017、D11S4463和D17S1301；多重体系VI：D5S2500、D9S1122和D21S1437）。计算了等位基因频率和法医学参数，以评估这些非联合DNA索引系统（CODIS）的微型STR系统的适用性和稳健性。除了D4S2364、D5S2500和D20S161位点外，未观察到与哈迪-温伯格平衡预期有显著偏差。基于6个微型STR位点（D1S1677、D2S441、D4S2364、D10S1248、D14S1434和D22S1045）的等位基因频率绘制的多维标度图显示，与这里比较的其他欧亚人群相比，韩国人与中国人和日本人似乎具有最高的遗传亲和力。由18个微型STR位点计算出的匹配联合概率为2.902×10⁻¹⁷，表明多态性程度很高。因此，这18个微型STR位点可适用于从降解的法医案件样本中获取有用信息，以及为涉及亲缘关系较近个体的各种分析提供补充遗传信息，这些分析需要额外的遗传信息。
