Asamura H, Uchida R, Takayanagi K, Ota M, Fukushima H
Department of Legal Medicine, Shinshu University School of Medicine, Matsumoto, Nagano, Japan.
Int J Legal Med. 2006 May;120(3):182-4. doi: 10.1007/s00414-005-0015-4. Epub 2005 Jul 13.
Allele frequencies and forensic parameters for the six miniSTR loci D1S1677, D2S441, D4S2364, D10S1248, D14S1434, and D22S1045 were investigated in a sample of 142 unrelated healthy Japanese individuals. The polymerase chain reaction (PCR) products contained within the six loci were less than 119 bp in size. The frequency distributions in the six short tandem repeat (STR) loci showed no deviations from Hardy-Weinberg equilibrium expectations. The accumulated powers of discrimination and power of exclusion for the six loci were 0.999998 and 0.98, respectively. It was thus considered that due to the small PCR products and the moderate degree of polymorphism, analysis with use of the six miniSTR loci was highly beneficial for the forensic analysis of degraded DNA.
在142名无亲缘关系的健康日本个体样本中，研究了6个微型短串联重复序列（miniSTR）基因座D1S1677、D2S441、D4S2364、D10S1248、D14S1434和D22S1045的等位基因频率和法医学参数。这6个基因座内的聚合酶链反应（PCR）产物大小均小于119 bp。6个短串联重复序列（STR）基因座的频率分布未显示出偏离哈迪-温伯格平衡预期的情况。这6个基因座的累积鉴别力和排除力分别为0.999998和0.98。因此，由于PCR产物较小且多态性程度适中，使用这6个miniSTR基因座进行分析对降解DNA的法医学分析非常有益。
