Hansen J L, McCutchan T F
Mol Biochem Parasitol. 1986 Apr;19(1):11-8. doi: 10.1016/0166-6851(86)90060-5.
Fragments of Plasmodium knowlesi DNA, generated by mung bean nuclease digestion, were ligated into the lambda gt11 vector. This expression library was immunoscreened with a serum which inhibits invasion of erythrocytes by merozoites in vitro and whose primary specificity is directed against a Mr 140 000 merozoite surface antigen. One of the isolated clones contained a 125 base pair insert which hybridized to a 1.8 kilobase species of schizont RNA, indicating that this insert is part of a gene expressed during schizogony.
由绿豆核酸酶消化产生的诺氏疟原虫DNA片段被连接到λgt11载体中。用一种血清对该表达文库进行免疫筛选，该血清在体外可抑制裂殖子对红细胞的侵袭，其主要特异性针对一种分子量为140 000的裂殖子表面抗原。其中一个分离的克隆含有一个125个碱基对的插入片段，它与一种1.8千碱基的裂殖体RNA杂交，表明该插入片段是裂体生殖期间表达的一个基因的一部分。
