Waters A P, Thomas A W, Deans J A, Mitchell G H, Hudson D E, Miller L H, McCutchan T F, Cohen S
Malaria Section, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, Maryland 20892.
J Biol Chem. 1990 Oct 15;265(29):17974-9.
The 66-kDa merozoite surface antigen (PK66) of Plasmodium knowlesi, a simian malaria, possesses vaccine-related properties that are thought to originate from a receptor-like role in parasite invasion of erythrocytes. We report the complete sequence of PK66 which allowed the demonstration that highly conserved analogues exist throughout Plasmodium including a recently reported gene from P. falciparum (Peterson, M. G., Marshall, V. M., Smythe, J. A., Crewther, P. E., Lew, A., Silva, A., Anders, R. F., and Kemp, D. J. (1989) Mol. Cell. Biol. 9, 3151-3155). These analogues are highly promising vaccination candidates. The distribution of PK66 changes after schizont rupture in a coordinate manner associated with merozoite invasion. The protein is concentrated at the apical end prior to rupture, following which it can distribute itself entirely across the surface of the free merozoite. During invasion, immunofluorescence studies suggest that, PK66 is excluded from the erythrocyte at, and behind, the invasion interface.
诺氏疟原虫（一种猿猴疟疾）的66-kDa裂殖子表面抗原（PK66）具有与疫苗相关的特性，这些特性被认为源于其在寄生虫侵入红细胞过程中类似受体的作用。我们报道了PK66的完整序列，这使得我们能够证明在整个疟原虫属中存在高度保守的类似物，包括最近报道的来自恶性疟原虫的一个基因（彼得森，M. G.，马歇尔，V. M.，斯迈思，J. A.，克鲁瑟，P. E.，卢，A.，席尔瓦，A.，安德斯，R. F.，和肯普，D. J.（1989年）《分子细胞生物学》9，3151 - 3155）。这些类似物是非常有前景的疫苗候选物。裂殖体破裂后，PK66的分布会以与裂殖子侵入相关的协调方式发生变化。在破裂之前，该蛋白集中在顶端，之后它可以完全分布在游离裂殖子的表面。在侵入过程中，免疫荧光研究表明，在侵入界面处及之后，PK66被排除在红细胞之外。
