Department of Digestive and Transplant Surgery, The University of Tokushima, Tokushima City, Tokushima, Japan.
Department of Digestive and Transplant Surgery, The University of Tokushima, Tokushima City, Tokushima, Japan.
J Surg Res. 2014 Apr;187(2):667-72. doi: 10.1016/j.jss.2013.10.031. Epub 2013 Oct 21.
Adipose tissue-derived stem cells (ADSCs), which are widely known as multipotent progenitor cells, release several cytokines that support cell survival and repair. The aim of this study was to investigate whether ADSC-secreted molecules could induce a trophic effect in pancreatic islet culture conditions in vitro.
We cocultured porcine islet cells with ADSCs using a transwell system for 48 h and evaluated the viability of islet cells. We also determined the concentration levels of cytokines and insulin in the supernatant of the culture medium. We used anti-vascular endothelial growth factor (VEGF) and anti-interleukin (IL)-6 receptor antibodies to investigate the effect of VEGF and IL-6 on islet cells.
ADSCs improved the viability of islet cells in the absence of cell-cell contact (P < 0.05). VEGF and IL-6 levels in the culture medium increased when islet cells were cocultured with ADSCs (P < 0.05). Furthermore, inhibition of VEGF decreased the viability of islet cells (P < 0.05); however, inhibition of IL-6 did not affect islet cell viability.
These results suggested that trophic factors, particularly VEGF, secreted by human ADSCs enhanced the survival and function of porcine islet cells.
脂肪组织来源的干细胞（ADSCs），作为多能祖细胞而广为人知，可分泌多种细胞因子以支持细胞存活和修复。本研究旨在探讨 ADSC 分泌的分子是否能在体外胰岛培养条件下诱导营养作用。
我们使用 Transwell 系统将猪胰岛细胞与 ADSC 共培养 48 小时，并评估胰岛细胞的活力。我们还测定了培养上清中细胞因子和胰岛素的浓度。我们使用抗血管内皮生长因子（VEGF）和抗白细胞介素（IL）-6 受体抗体来研究 VEGF 和 IL-6 对胰岛细胞的影响。
ADSC 在无细胞-细胞接触的情况下改善了胰岛细胞的活力（P < 0.05）。当胰岛细胞与 ADSC 共培养时，培养基中 VEGF 和 IL-6 的水平增加（P < 0.05）。此外，抑制 VEGF 降低了胰岛细胞的活力（P < 0.05）；然而，抑制 IL-6 并不影响胰岛细胞的活力。
这些结果表明，人 ADSC 分泌的营养因子，特别是 VEGF，增强了猪胰岛细胞的存活和功能。
