Qin Bolin, Panickar Kiran S, Anderson Richard A
Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD, USA; Integrity Nutraceuticals International, Spring Hill, TN, USA.
Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD, USA; Department of Pediatrics, University of Maryland School of Medicine, Baltimore, MD, USA.
Nutrition. 2014 Feb;30(2):210-7. doi: 10.1016/j.nut.2013.07.001. Epub 2013 Nov 12.
Increasing evidence suggests that cinnamon has many health benefits when used in herbal medicine and as a dietary ingredient. The aim of this study was to investigate the effects of an aqueous extract of cinnamon, high in type A polyphenols, on molecular targets in rat C6 glioma cells that underlie their protective effects.
C6 rat glioma cells were seeded in 35-mm culture dishes or six-well plates, then were incubated with cinnamon polyphenols at doses of 10 and 20 μg/mL for 24 h. The targeting protein expression, secretion, and phosphorylation were evaluated by immunoprecitation/immunoblotting and immunofluorescence imaging.
Cinnamon polyphenols significantly enhanced secretion of S100β, a Ca(2+)-binding protein, and increased intracellular S100β expression after 24 h of incubation, in rat C6 glioma cells. Cinnamon polyphenols also enhanced protein levels of sirtuin 1, 2, and 3, deacetylases important in cell survival, and the tumor suppressor protein, p53, and inhibited the inflammatory factors, tumor necrosis factor alpha, and phospho-p65, a subunit of nuclear factor-κβ. Cinnamon polyphenols also up-regulated levels of phospho-p38, extracellular signal-regulated protein and mitogen-activated protein and kinase-activated protein kinases that may be important for prosurvival functions.
Our results indicate that the effects of cinnamon polyphenols on upregulating prosurvival proteins, activating mitogen-activated protein kinase pathways, and decreasing proinflammatory cytokines may contribute to their neuroprotective effects.
越来越多的证据表明，肉桂作为草药和膳食成分使用时具有多种健康益处。本研究的目的是调查富含A型多酚的肉桂水提取物对大鼠C6胶质瘤细胞中构成其保护作用基础的分子靶点的影响。
将C6大鼠胶质瘤细胞接种于35mm培养皿或六孔板中，然后用10和20μg/mL剂量的肉桂多酚孵育24小时。通过免疫沉淀/免疫印迹和免疫荧光成像评估靶向蛋白的表达、分泌和磷酸化。
在大鼠C6胶质瘤细胞中，肉桂多酚孵育24小时后，显著增强了Ca(2+)结合蛋白S100β的分泌，并增加了细胞内S100β的表达。肉桂多酚还提高了对细胞存活至关重要的去乙酰化酶sirtuin 1、2和3以及肿瘤抑制蛋白p53的蛋白水平，并抑制了炎症因子肿瘤坏死因子α和核因子κβ亚基磷酸化p65。肉桂多酚还上调了磷酸化p38、细胞外信号调节蛋白和丝裂原活化蛋白以及激酶活化蛋白激酶的水平，这些可能对促生存功能很重要。
我们的结果表明，肉桂多酚上调促生存蛋白、激活丝裂原活化蛋白激酶途径以及减少促炎细胞因子的作用可能有助于其神经保护作用。
