Uphouse L, Eckols K, Sierra V, Kolodziej M, Brown H
Neurotoxicology. 1986 Spring;7(1):127-41.
The effect of chlordecone on behavioral estrus was examined in adult ovariectomized female rats. Chlordecone has been reported to resemble estrogen in altering pituitary secretions, in producing vaginal cornification, in increasing uterine weight and in competing for binding to the estrogen receptor. In the present study, 10, 25, 50 or 75 mg/kg chlordecone was substituted for estrogen in the estrogen-progesterone priming sequence used to facilitate sexual behavior of ovariectomized female rats. Sexual receptivity was measured by the number of lordosis responses exhibited by the female when mounted by a sexually active male. Chlordecone failed to substitute for estradiol in producing lordosis behavior. When female rats were given chlordecone in addition to estrogen plus progesterone, chlordecone reduced the lordosis behavior usually seen in these steroid primed animals. In further studies, chlordecone's effect on the CNS progesterone receptor was examined. Unlike estradiol, chlordecone did not induce progesterone receptors. Furthermore, chlordecone attenuated the increase in progesterone receptors seen after estradiol treatment. These findings suggest that chlordecone fails to mimic, and may actually interfere with, estrogen's facilitative effects on neurally mediated reproductive events.
在成年去卵巢雌性大鼠中研究了开蓬对行为性发情的影响。据报道，开蓬在改变垂体分泌、产生阴道角质化、增加子宫重量以及竞争与雌激素受体结合方面类似于雌激素。在本研究中，用10、25、50或75mg/kg的开蓬替代雌激素-孕酮启动序列中的雌激素，该序列用于促进去卵巢雌性大鼠的性行为。通过有性活动的雄性骑跨时雌性表现出的脊柱前凸反应数量来测量性接受能力。开蓬在产生脊柱前凸行为方面不能替代雌二醇。当给雌性大鼠同时给予开蓬以及雌激素加孕酮时，开蓬降低了这些用类固醇启动的动物中通常可见的脊柱前凸行为。在进一步的研究中，检测了开蓬对中枢神经系统孕酮受体的影响。与雌二醇不同，开蓬不会诱导孕酮受体。此外，开蓬减弱了雌二醇治疗后可见的孕酮受体增加。这些发现表明，开蓬无法模拟，实际上可能会干扰雌激素对神经介导的生殖事件的促进作用。
