Department of Life Sciences, Faculty of Science, Okayama University of Science, 1-1 Ridai-cho, Kita-ku, Okayama-shi, Okayama 700-0005, Japan.
Department of Life Sciences, Faculty of Science, Okayama University of Science, 1-1 Ridai-cho, Kita-ku, Okayama-shi, Okayama 700-0005, Japan.
Anaerobe. 2014 Feb;25:67-71. doi: 10.1016/j.anaerobe.2013.11.002. Epub 2013 Nov 15.
The adhesive properties of Clostridium perfringens to collagens, gelatin, fibronectin (Fn), Fn-prebound collagens, and Fn-prebound gelatin were investigated. C. perfringens could bind to Fn-prebound collagen type II, type III, and gelatin, but not to gelatin or collagens except for collagen type I directly. Recombinant Fn-binding proteins of C. perfringens, rFbpA and rFbpB, were used to examine Fn-mediated bacterial adherence to collagen type I. In the presence of rFbps, C. perfringens adherence to Fn-prebound collagen type I was inhibited in a dose-dependent manner. Fn was not released from the coated collagen type I by the presence of rFbps, and rFbps did not bind to collagen type I. Thus, the inhibition of C. perfringens binding to Fn-prebound collagen type I by rFbps could not be explained by the removal of Fn from collagen or by the competitive binding of rFbps to collagen. Instead, both rFbps were found to bind to C. perfringens. These results suggest the possibility that rFbps may bind to the putative Fn receptor expressed on C. perfringens and competitively inhibit Fn binding to C. perfringens.
研究了产气荚膜梭菌对胶原蛋白、明胶、纤维连接蛋白（Fn）、Fn 结合胶原蛋白和 Fn 结合明胶的黏附特性。产气荚膜梭菌可以结合到 Fn 结合的 II 型、III 型胶原蛋白和明胶上，但不能结合到明胶或胶原蛋白上，除了直接结合 I 型胶原蛋白。使用产气荚膜梭菌的重组 Fn 结合蛋白 rFbpA 和 rFbpB 来检测 Fn 介导的细菌对胶原蛋白 I 的黏附。在 rFbps 的存在下，产气荚膜梭菌对 Fn 结合的胶原蛋白 I 的黏附被剂量依赖性抑制。Fn 没有被 rFbps 从涂层的胶原蛋白 I 上释放出来，并且 rFbps 也没有与胶原蛋白 I 结合。因此，rFbps 对 Fn 结合的胶原蛋白 I 与产气荚膜梭菌结合的抑制不能用 Fn 从胶原蛋白上的去除或 rFbps 与胶原蛋白的竞争结合来解释。相反，两种 rFbps 都被发现与产气荚膜梭菌结合。这些结果表明 rFbps 可能与产气荚膜梭菌上表达的假定 Fn 受体结合，并竞争性抑制 Fn 与产气荚膜梭菌的结合。
