Sun Zhifeng, Lu Mingmin, Lillehoj Hyun, Lee Youngsub, Goo Doyun, Yuan Baohong, Yan Xianghe, Li Charles
Animal Bioscience and Biotechnology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, US Department of Agriculture, Beltsville, MD 20705, USA.
Environment Microbial and Food Safety Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, US Department of Agriculture, Beltsville, MD 20705, USA.
Pathogens. 2023 May 30;12(6):778. doi: 10.3390/pathogens12060778.
is the etiological agent for necrotic enteritis (NE) in broiler chickens, which causes a substantial economic loss of an estimated USD 6 billion annually in the global poultry industry. Collagen adhesion is involved in the NE pathogenesis in poultry. In this study, the binding capabilities of chicken isolates of various genetic backgrounds (, , ) to collagen types I-V and gelatin were examined, and the putative adhesin protein gene was investigated at the genomic level. In total, 28 strains from healthy and NE-inflicted sick chickens were examined. The results on collagen adhesin-encoding gene by the quantitative-PCR results indicated that isolates had much lower copies of the detectable gene than isolates (10 , 5 isolates). Most of the virulent isolates demonstrated collagen-binding abilities to types I-II and IV-V, while some strains showed weak or no binding to collagen type III and gelatin. However, the isolates showed significantly higher binding capabilities to collagen III than and isolates. The data in this study suggest that the collagen-binding capability of clinical isolates correlates well with their NE pathogenicity levels, especially for isolates carrying genes encoding crucial virulence factors and virulence-associated factors such as , and . These results indicate that the presence of the gene may be correlated with virulence (particularly for isolates).
是肉鸡坏死性肠炎（NE）的病原体，在全球家禽业中每年造成约60亿美元的重大经济损失。胶原蛋白黏附参与家禽NE的发病机制。在本研究中，检测了不同遗传背景（、、）的鸡分离株对I-V型胶原蛋白和明胶的结合能力，并在基因组水平上研究了假定的黏附蛋白基因。总共检测了来自健康鸡和患NE病鸡的28株菌株。定量PCR结果显示，分离株中可检测到的基因拷贝数比分离株低得多（10，5株分离株）。大多数强毒分离株对I-II型和IV-V型胶原蛋白表现出结合能力，而一些菌株对III型胶原蛋白和明胶的结合较弱或无结合。然而，分离株对III型胶原蛋白的结合能力明显高于和分离株。本研究数据表明，临床分离株的胶原蛋白结合能力与其NE致病水平密切相关，特别是对于携带编码关键毒力因子和毒力相关因子（如、和）基因的分离株。这些结果表明，基因的存在可能与毒力相关（特别是对于分离株）。
