Division of Marine Environment & BioScience, Korea Maritime and Ocean University, Busan 606-791, Republic of Korea.
Department of Biological Sciences, University of Calgary, 2500 University Drive N.W. Calgary, Alberta T3B 2V4, Canada.
Comp Biochem Physiol A Mol Integr Physiol. 2014 Feb;168:17-24. doi: 10.1016/j.cbpa.2013.11.003. Epub 2013 Nov 13.
While there have been a number of studies on the effects of photoperiod and duration of light and dark exposure, much less information is available on the importance of light intensity. This study investigated the effects of exposure of goldfish, Carassius auratus exposed to white fluorescent bulbs, and red (peak at 630nm), and green (530nm) light emitting diodes (LEDs) at approximately 0.9W/m(2) (12-h light:12-h dark) for four months on a number of hormones of the hypothalamus-pituitary-gonad (HPG) axis, in vivo and in vitro. We investigated the effects of native GnRH molecules (gonadotropin-releasing hormones; salmon GnRH, sGnRH; and chicken GnRH-II, cGnRH-II), gonadotropin hormones (GTHα; follicle-stimulating hormone, FSH-β; luteinizing hormone, LH-β2), kisspeptin 1 (Kiss1) and G protein-coupled receptor 54 (GPR54) mRNA levels. Furthermore, we measured LH and 17α-hydroxypregnenolone levels in plasma and we performed gonad histological observations. GnRHs, Kiss1, GPR54 and GTH mRNA and plasma LH and 17α-hydroxypregnenolone levels in the in vivo and in vitro groups exposed to green LEDs were significantly higher than the other groups. Histological analysis revealed the presence of oocytes in the yolk stage in fish exposed to green light. These results suggest that green wavelengths regulate the HPG axis and enhance sexual maturation in goldfish.
虽然已经有许多关于光周期和明暗持续时间对鱼类影响的研究，但关于光强度重要性的信息却很少。本研究调查了金鱼（Carassius auratus）暴露于白色荧光灯泡以及红色（峰值在 630nm）和绿色（530nm）发光二极管（LED）下，在 0.9W/m²（12 小时光照：12 小时黑暗）的环境中 4 个月对下丘脑-垂体-性腺（HPG）轴的多种激素的影响，包括体内和体外实验。我们研究了内源性 GnRH 分子（促性腺激素释放激素；鲑鱼 GnRH，sGnRH；和鸡 GnRH-II，cGnRH-II）、促性腺激素（GTHα；卵泡刺激素，FSH-β；黄体生成素，LH-β2）、kisspeptin 1（Kiss1）和 G 蛋白偶联受体 54（GPR54）mRNA 水平的影响。此外，我们测量了血浆中的 LH 和 17α-羟孕烯醇酮水平，并进行了性腺组织学观察。暴露于绿光的体内和体外实验组的 GnRHs、Kiss1、GPR54 和 GTH mRNA 以及血浆 LH 和 17α-羟孕烯醇酮水平均显著高于其他组。组织学分析显示，暴露于绿光的鱼体内存在卵黄期的卵母细胞。这些结果表明，绿光波长调节 HPG 轴并促进金鱼的性成熟。
