Song Jin Ah, Kim Na Na, Choi Young Jae, Choi Ji Yong, Kim Bong-Seok, Choi Cheol Young
Division of Marine BioScience, Korea Maritime and Ocean University, Busan, 49112, South Korea.
Jeju Fisheries Research Institute, National Institute of Fisheries Science, Jeju, 63068, South Korea.
Biochem Biophys Res Commun. 2016 Aug 5;476(4):541-545. doi: 10.1016/j.bbrc.2016.05.159. Epub 2016 May 30.
We determined the molecular mechanism underlying the environmental (photoperiodic) regulation of sexual maturation in fish, we examined the expression of sexual maturation-related hormones and vertebrate ancient long opsin (VAL-opsin) in goldfish (Carassius auratus) exposed to different light spectra (red and green light-emitting diodes). We further evaluated the effect of exogenous gonadotropin hormone (GTH) on the expression of VAL-opsin under different light conditions. Our results demonstrated that the expression of GTHs was higher in the fish exposed to green light, and VAL-opsin levels were increased in the fish receiving GTH injection. Therefore, we have uncovered a molecular mechanism underlying the environmental (light)-induced trigger for sexual maturation: VAL-opsin is activated by green light and GTH, which promotes the expression of sexual maturation genes.
我们确定了鱼类性腺成熟的环境（光周期）调节的分子机制，研究了暴露于不同光谱（红色和绿色发光二极管）下的金鱼（Carassius auratus）中性腺成熟相关激素和脊椎动物古老长视蛋白（VAL-视蛋白）的表达。我们进一步评估了外源促性腺激素（GTH）在不同光照条件下对VAL-视蛋白表达的影响。我们的结果表明，暴露于绿光下的鱼体内促性腺激素的表达较高，接受GTH注射的鱼体内VAL-视蛋白水平增加。因此，我们揭示了环境（光）诱导性腺成熟触发的分子机制：VAL-视蛋白被绿光和GTH激活，从而促进性腺成熟基因的表达。
