Department of Biology, Centre for Biomedical Research, University of Victoria, Victoria, British Columbia V8W 3N5, Canada; Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada.
Department of Biology, Electron Microscopy Laboratory, University of Victoria, Victoria, British Columbia V8W 3N5, Canada.
Comp Biochem Physiol B Biochem Mol Biol. 2014 Feb;168:33-44. doi: 10.1016/j.cbpb.2013.11.004. Epub 2013 Nov 13.
The products of dax1, foxl2a and mis have each been shown to have proliferative and/or differentiative activities during mammalian organogenesis. These factors also play a role in regulating the biosynthesis of estrogen, particularly by modulating the activity of aromatase cyp19a. We demonstrate the transcription and translation of these genes during salmon embryogenesis. We were able to track sex-specific differences in these processes through accurate determination of the sex of each embryo and larva examined from genotyped microsatellites. We detected sex- and stage-specific immunolabeling of the embryonic gut, kidney, gonads, neural cord and skeletal muscle by DAX-1, FOXL2A and MIS. These results indicate the potential of these factors to mediate proliferation and/or differentiation programs during development of these tissues. As well, immunolabeling of skeletal muscle by CYP19B1 throughout the study reveals probable neurogenic activity associated with peripheral radial glial cells and the growing embryonic musculature.
在哺乳动物器官发生过程中，dax1、foxl2a 和 mis 的产物都表现出增殖和/或分化活性。这些因子在调节雌激素的生物合成中也发挥作用，特别是通过调节细胞色素 P45019A（aromatase cyp19a）的活性。我们在鲑鱼胚胎发生过程中证明了这些基因的转录和翻译。通过对从基因分型微卫星中检查的每个胚胎和幼虫的准确性别确定，我们能够跟踪这些过程中的性别特异性差异。我们通过 DAX-1、FOXL2A 和 MIS 检测到胚胎肠道、肾脏、性腺、脊索和骨骼肌的性别和阶段特异性免疫标记。这些结果表明这些因子在这些组织的发育过程中具有介导增殖和/或分化程序的潜力。此外，在整个研究过程中，CYP19B1 对骨骼肌的免疫标记揭示了与周围放射状神经胶质细胞和发育中的胚胎肌肉组织相关的可能的神经发生活性。
