高效液相色谱蒸发光散射检测和电喷雾串联质谱法分析微藻中的中性脂
Analysis of neutral lipids from microalgae by HPLC-ELSD and APCI-MS/MS.
机构信息
UMR Qualisud (CIRAD, Université Montpellier II, UM1, Sup Agro), Place E. Bataillon, 34095 Montpellier Cedex 5, France; Total SA, PERL-Pôle d'Etudes et de Recherche de Lacq, BP 47, 64170 Lacq, France.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Dec 30;942-943:98-106. doi: 10.1016/j.jchromb.2013.10.016. Epub 2013 Oct 21.
A method was developed to analyze neutral lipids through the use of three triglycerides, four free fatty acids, six di- and four mono-glycerides standards by high performance liquid chromatography (HPLC) normal phase coupled with either with evaporative light scattering detector (ELSD) or with mass spectrometry (MS) operating in atmospheric pressure chemical ionization (APCI) mode. The method was applied to the determination of the neutral lipid fraction from a Botryococcus braunii race A (B. braunii) culture. This method led us to identify neutral lipids synthesized by B. braunii in a single analysis within 45min through HPLC-APCI-MS/MS technique.
开发了一种通过高效液相色谱（HPLC）正相结合蒸发光散射检测器（ELSD）或大气压化学电离（APCI）模式下的质谱（MS）分析三种三酸甘油酯、四种游离脂肪酸、六种二脂和四种单脂标准品来分析中性脂质的方法。该方法应用于从 Botryococcus braunii 种 A（B. braunii）培养物中测定中性脂质部分。通过 HPLC-APCI-MS/MS 技术，该方法使我们能够在 45 分钟内对 B. braunii 合成的中性脂质进行单一分析。
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