Plant Breeding Institute, Maris Lane, Trumpington, CB2 2LQ, Cambridge, UK.
Planta. 1986 Mar;167(3):344-50. doi: 10.1007/BF00391337.
The specific activity of ribulose-1,5-bisphosphate carboxylase (RuBPCase; EC 4.1.1.39) in crude extracts of leaves from euploid, amphiploid and alloplasmic lines of wheat fell into high or low categories (3.75 or 2.70 μmol·mg(-1)·min(-1), 30°C). For the alloplasmic lines, where the same hexaploid nuclear genome was substituted into different cytoplasms, the specific activity of RuBPCase was consistent with the type of cytoplasm (high for the B and S cytoplasms and low for the A and D cytoplasms). There was no evidence from the euploid and amphiploid lines that small subunits encoded in different nuclear genomes influenced the specific activity. High specific activity was conferred by possession of the chloroplast genome of the B-type cytoplasm which encodes the large subunit of RuBPCase. All lines with a cytoplasm derived from the Sitopsis section of wheat, with the exception of Aegilops longissima and A. speltoides 18940, had RuBPCase with high specific activity. In contrast with the euploid lines of A. longissima, the alloplasmic line containing A. longissima cytoplasm from a different source had RuBPCase with high specific activity. The difference in specific activity found here in-vitro was not apparent in-vivo when leaf gas exchange was measured.
在小麦的同源多倍体、异源多倍体和异质体叶片粗提物中，核酮糖-1,5-二磷酸羧化酶（RuBPCase；EC 4.1.1.39）的比活落入高或低两个范畴（3.75 或 2.70 μmol·mg(-1)·min(-1)，30°C）。对于异质体，相同的六倍体核基因组被替代到不同的细胞质中，RuBPCase 的比活与细胞质的类型一致（B 和 S 细胞质的比活高，A 和 D 细胞质的比活低）。在同源多倍体和异源多倍体中，没有证据表明不同核基因组编码的小亚基会影响比活。B 型细胞质的叶绿体基因组编码 RuBPCase 的大亚基，高比活是由其拥有而赋予的。除了节节麦和长穗偃麦草 18940 之外，所有来自小麦 Sitopsis 组的细胞质的异质体都具有高比活的 RuBPCase。与节节麦的同源多倍体不同，含有不同来源的节节麦细胞质的异质体具有高比活的 RuBPCase。当测量叶片气体交换时，体内发现的比活差异在体外并不明显。
