Nuclear Agriculture Division, Bhabha Atomic Research Centre, 400 085, Trombay, Bombay, India.
Theor Appl Genet. 1986 Nov;73(1):82-7. doi: 10.1007/BF00273723.
Variation in the arachin polypeptides of groundnut genotypes was observed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Three regions could be observed on the electropherogram. Region 1, corresponding to conarachin, did not show any variation; region 2, consisting of arachin acidic subunits, showed variation; region 3, containing the arachin basic subunits, did not show any variation. There are four varietal classes of arachin polypeptide patterns: class A comprised three acidic subunits of arachin of molecular weights 47.5, 45.1 and 42.6 kd and a basic subunit of 21.4 kd; class B, with three acidic subunits of molecular weights 47.5, 45.1 and 41.2 kd and a basic subunit of 21.4 kd; class C of an additive pattern of class A and class B; class D, of two acidic polypeptides of 47.5, 45.1 kd and the basic 21.4 kd subunit. Of the 90 genotypes studied, 73% belong to class A, 15% to class B and 6% each to class C and D. Analysis of F2 seeds from a cross between class A and class B genotypes showed that the two polypeptides (42.6 kd and 41.2 kd) are coded by nonallelic genes and also revealed that class C and class D patterns arose as a result of hybridisation between class A and class B. A. monticola, the progenitor of A. hypogaea, showed a pattern similar to the additive pattern of class A and class B while some diploid Arachis species had the 41.2 kd polypeptide. Based on arachin polypeptide patterns the probable origin of A. hypogaea has been suggested.
通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）观察到花生基因型的 arachin 多肽的变化。在电泳图谱上可以观察到三个区域。区域 1，对应 conarachin，没有显示任何变化；区域 2，由 arachin 酸性亚基组成，显示出变化；区域 3，含有 arachin 碱性亚基，没有显示任何变化。arachin 多肽图谱有四个品种类别：A 类由分子量为 47.5、45.1 和 42.6 kd 的 arachin 三个酸性亚基和分子量为 21.4 kd 的碱性亚基组成；B 类，由分子量为 47.5、45.1 和 41.2 kd 的三个 arachin 酸性亚基和分子量为 21.4 kd 的碱性亚基组成；A 类和 B 类的加性模式 C 类；D 类，由分子量为 47.5、45.1 kd 的两个酸性多肽和基本的 21.4 kd 亚基组成。在所研究的 90 个基因型中，73%属于 A 类，15%属于 B 类，6%分别属于 C 类和 D 类。对 A 类和 B 类基因型杂交 F2 种子的分析表明，两个多肽（42.6 kd 和 41.2 kd）由非等位基因编码，还表明 C 类和 D 类模式是 A 类和 B 类杂交的结果。A. monticola，A. hypogaea 的祖先，表现出类似于 A 类和 B 类加性模式的模式，而一些二倍体 Arachis 物种具有 41.2 kd 多肽。基于 arachin 多肽模式，推测了 A. hypogaea 的可能起源。
