Lehrstuhl für Genetik, Institut für Biologie II, Universität Tübingen, D-7400, Tübingen, Federal Republic of Germany.
Theor Appl Genet. 1986 Nov;73(1):129-35. doi: 10.1007/BF00273729.
The AT-rich highly repeated satellite DNA of Cucurbita pepo (zucchini) and Cucurbita maxima (pumpkin) were cloned and their DNA structure was investigated. DNA sequencing revealed that the repeat length of satellite DNA in Cucurbita pepo is 349-352 base pairs. The percentage of AT-base pairs is about 61%. This satellite is highly conserved in restriction enzyme pattern and DNA sequence; sequence heterogeneity is about 10%. In contrast, the satellite DNA of Cucurbita maxima has a repeat length of 168-169 base pairs. This satellite is also rich in AT-base pairs (64%), existing in at least three different variants as revealed by restriction enzyme analysis and DNA sequencing. The sequence heterogeneity between these variants is about 15%. The two satellite DNAs showed no cross-hybridization to each other and sequence homology is only limited. Nevertheless, we found in the C. pepo genome a high amount of sequences resembling the satellite of C. maxima. In contrast, the satellite repeat of C. pepo is found in the C. maxima DNA only in a few copies. These observations were discussed with respect to satellite DNA evolution and compared to the data received from monocotyledonous species.
南瓜（西葫芦）和南瓜（南瓜）的富含 AT 的高度重复卫星 DNA 已被克隆，并对其 DNA 结构进行了研究。DNA 测序表明，西葫芦卫星 DNA 的重复长度为 349-352 个碱基。AT 碱基对的百分比约为 61%。该卫星在限制酶图谱和 DNA 序列上高度保守；序列异质性约为 10%。相比之下，南瓜的卫星 DNA 重复长度为 168-169 个碱基。该卫星也富含 AT 碱基对（64%），通过限制酶分析和 DNA 测序揭示了至少三种不同的变体。这些变体之间的序列异质性约为 15%。这两种卫星 DNA 彼此之间没有交叉杂交，序列同源性也很有限。尽管如此，我们在 C. pepo 基因组中发现了大量类似于 C. maxima 卫星的序列。相反，C. maxima DNA 中 C. pepo 的卫星重复仅存在少数几个拷贝。这些观察结果与卫星 DNA 进化有关，并与单子叶植物物种的数据进行了比较。
