Laboratoire de Biologie Cellulaire, I.N.R.A., F-78000, Versailles, France.
Theor Appl Genet. 1986 Dec;73(2):299-304. doi: 10.1007/BF00289289.
The use of increasing knowledge on regulation of nitrate reductase activity in Nicotiana cell cultures is the basis for the described optimization of in vitro selection for nitrate reductase-deficient mutants by screening for chlorate resistance. Selection was carried out on haploid mesophyll protoplast-derived cell cultures of Nicotiana plumbaginifolia. It is demonstrated that revised selection results in high variant detectability and increased variant confirmability in comparison with the hitherto used selection scheme.
利用不断增加的关于硝酸还原酶活性调控的知识，对烟草原生质体培养物中的硝酸还原酶缺失突变体进行了优化，通过筛选对氯酸盐的抗性进行体外选择。选择是在菫叶烟草的单倍体叶肉原生质体衍生细胞培养物上进行的。结果表明，与迄今使用的选择方案相比，修订后的选择方案可提高变异体的可检测性和可确认性。
