Heller M W, Stoddard S L
Stain Technol. 1986 Mar;61(2):71-3. doi: 10.3109/10520298609110710.
The described method provides a new technique for differentiating areas of gray and white matter in fixed human brain slices. The technique is a modification of an existing method permitting use of the nonfading copper phthalocyanine dye alcian blue. Stained slices show turquoise gray matter that contrasts sharply with areas of white matter.
Cut human brains from gross anatomy laboratory cadavers into 4 mm slices and wash in running tap water for 12 hr. Oxidize slices in performic acid for 1.5 hr. Wash in running tap water for 12 hr. Stain slices in shallow dishes in 0.05% aqueous alcian blue. Wash in running tap water for 1 hr. Dry for 2-4 hr and embed in plastic.
所描述的方法为区分固定人脑切片中的灰质和白质区域提供了一种新技术。该技术是对现有方法的改进，允许使用不褪色的铜酞菁染料阿尔辛蓝。染色后的切片显示出蓝绿色的灰质，与白质区域形成鲜明对比。
从大体解剖实验室尸体上切下人脑，切成4毫米厚的切片，在流动的自来水中冲洗12小时。在过甲酸中氧化切片1.5小时。在流动的自来水中冲洗12小时。将切片放在浅盘中用0.05%的阿尔辛蓝水溶液染色。在流动的自来水中冲洗1小时。干燥2 - 4小时，然后嵌入塑料中。
