National Institute of Agrobiological Resources, 305, Tsukuba Science City, Ibaraki, Japan.
Plant Cell Rep. 1988 May;7(3):153-7. doi: 10.1007/BF00269311.
A continuous flow electro-manipulator available both for mass production of fused and of transfected plant protoplasts was devised using a flow chamber with gold-coated glass panel electrodes. Up to 100 ml of protoplasts suspension were treated within 20 min at the rate of approximately 1×10(6) protoplasts / min. The yield of diheterokaryons between tobacco mesophyll and carrot root protoplasts reached approximately 10 % of total protoplasts by flow electro-fusion. More than 95 % of tobacco and cowpea mesophyll protoplasts became infected with tobacco mosaic virus RNA by flow electro-transfection.
一种连续流动电动操作器，可用于融合和转染植物原生质体的大规模生产，它使用带有镀金玻璃面板电极的流动室设计而成。在 20 分钟内，可处理高达 100 毫升的原生质体悬浮液，速度约为每分钟 1×10(6)个原生质体。通过流动电融合，烟草叶肉和胡萝卜根原生质体之间的异核体产量达到总原生质体的约 10%。通过流动电转染，超过 95%的烟草和豇豆叶肉原生质体被烟草花叶病毒 RNA 感染。
