Rhodes M B, Baker P K
Vet Parasitol. 1986 Apr;20(4):333-40. doi: 10.1016/0304-4017(86)90130-5.
The sensitivity of an indirect radioimmunoassay (IRIA) used for detecting larval body wall (LBW) antigens of Ascaris suum was enhanced by using various blocking agents which prevented nonspecific binding of immunoglobulin G (IgG) or free 125I without preventing binding of specific antibodies to the antigen. The use of blocking agents reduced counts for both positive and negative sera, resulting in an increase in calculated binding ratios (BR) and deltas and, thus, in the sensitivity of the assay. The relative effectiveness of blocking agents, in decreasing order, were turkey serum (TS), rabbit gamma globulin (RGG), rabbit whole serum (RS), bovine serum albumin (BSA), rabbit IgG (RIgG), bovine alpha globulin (BAG), bovine gamma globulin (BGG) and Tween-20.
通过使用各种封闭剂提高了用于检测猪蛔虫幼虫体壁（LBW）抗原的间接放射免疫分析（IRIA）的灵敏度，这些封闭剂可防止免疫球蛋白G（IgG）或游离的125I发生非特异性结合，同时又不阻止特异性抗体与抗原的结合。封闭剂的使用降低了阳性和阴性血清的计数，导致计算出的结合率（BR）和δ值增加，从而提高了检测的灵敏度。封闭剂的相对有效性由高到低依次为火鸡血清（TS）、兔γ球蛋白（RGG）、兔全血清（RS）、牛血清白蛋白（BSA）、兔IgG（RIgG）、牛α球蛋白（BAG）、牛γ球蛋白（BGG）和吐温-20。
