一种针对重组人粒细胞集落刺激因子（rhG）的人源单克隆抗体。

A human monoclonal antibody to rhG.

作者信息

Foung S K, Blunt J, Perkins S, Winn L, Grumet F C

出版信息

Vox Sang. 1986;50(3):160-3. doi: 10.1111/j.1423-0410.1986.tb04870.x.

DOI:10.1111/j.1423-0410.1986.tb04870.x

Abstract

Hybridomas secreting a human monoclonal IgG1 antibody against a variant of the rhG antigen were produced from B lymphocytes of an Rh-negative donor with serum antibodies to D and G [correction of C] antigens. The antibody reacts by indirect hemagglutination with nearly all C- or D-positive cells, confirming the strong association in the expression of G with D or C antigens. The lack of antibody reactivity to C-negative Du cells suggests a particular epitope on the D complex associated with the G antigen.

摘要

利用一名Rh阴性供体的B淋巴细胞制备了分泌抗rhG抗原变体的人单克隆IgG1抗体的杂交瘤，该供体血清中含有针对D和G [校正为C]抗原的抗体。该抗体通过间接血凝反应与几乎所有C或D阳性细胞发生反应，证实了G抗原表达与D或C抗原之间存在强关联。对C阴性Du细胞缺乏抗体反应性表明D复合物上存在与G抗原相关的特定表位。