一种针对高频红细胞抗原Jra的人单克隆抗体。

A human monoclonal antibody to high-frequency red cell antigen Jra.

作者信息

Miyazaki T, Kwon K W, Yamamoto K, Tone Y, Ihara H, Kato T, Ikeda H, Sekiguchi S

机构信息

Hokkaido Red Cross Blood Center, Sapporo, Japan.

出版信息

Vox Sang. 1994;66(1):51-4. doi: 10.1111/j.1423-0410.1994.tb00277.x.

DOI:10.1111/j.1423-0410.1994.tb00277.x

PMID:8146983

Abstract

A human-mouse heterohybridoma (HMR0921) secreting human monoclonal IgG3, lambda antibody was produced from peripheral blood lymphocytes of a healthy blood donor with serum antibody to Jra, by EBV transformation and hybridization with mouse myeloma cell line P3X63Ag8.653. The reactivity of HMR0921 antibody was assessed by antiglobulin test with a panel of red cells including 14 different rare blood types. Only Jr(a-) red cells were negative. The strict specificity of this antibody to Jra antigen was further confirmed by absorption test with fluorescence flow cytometry. On screening of 28,744 blood donor samples by HMR0921 antibody, we detected 19 agglutination-negative samples, which were confirmed as Jr(a-) by conventional anti-Jra antisera. Therefore, our HMR0921 antibody is extremely useful for detecting rare Jr(a-) blood.

摘要

用人EB病毒转化健康献血者外周血淋巴细胞，并与小鼠骨髓瘤细胞系P3X63Ag8.653杂交，制备出分泌人单克隆IgG3、λ抗体的人-鼠杂种瘤（HMR0921），该献血者血清中含有针对Jr(a)的抗体。用一组包括14种不同稀有血型的红细胞通过抗球蛋白试验评估HMR0921抗体的反应性。只有Jr(a-)红细胞呈阴性。通过荧光流式细胞术吸收试验进一步证实了该抗体对Jr(a)抗原的严格特异性。用HMR0921抗体筛查28744份献血者样本时，我们检测到19份凝集阴性样本，经传统抗Jr(a)抗血清确认为Jr(a-)。因此，我们的HMR0921抗体对于检测罕见的Jr(a-)血型极为有用。