盐草（Distichlis spicata）悬浮培养中外源脯氨酸的生长抑制及其代谢。

Growth inhibition by exogenous proline and its metabolism in saltgrass (Distichlis spicata) suspension cultures.

机构信息

Genetics Group (LS-3), Mail Stop M886, Life Science Division, Los Alamos National Laboratory, 87545, Los Alamos, NM, USA.

出版信息

Plant Cell Rep. 1988 Aug;7(5):305-8. doi: 10.1007/BF00269924.

DOI:10.1007/BF00269924

PMID:24241870

Abstract

The growth of Distichlis spicata suspension cultures in LS medium without NaCl was inhibited 54% by 2 mM proline. In medium containing 260 mM NaCl, 10 mM proline inhibited growth by only 22%. The uptake and metabolism of 10 mM L-[1-(13)C] proline was followed by (13)C NMR and ninhydrin analyses of suspensions cultured in the presence of 0 or 260 mM NaCl. Uptake of 85 to 92% of the exogenous proline occurred within 72 h in all media. In 10 mM proline and no NaCl, cellular proline reached a maximm of 51.5 μmoles/g FW compared to 1.9 μmoles/g FW in suspensions not grown on proline. In medium containing 260 mM NaCl and proline, cellular proline reached 59-65 μmoles/g FW compared to 30-40 μmoles/g FW in controls grown without proline. The (13)C-label in the proline-C1 was either retained in proline or disappeared, presumably released as carbon dioxide, by catabolism through the TCA cycle. Since no metabolite of (13)C-proline was detected by NMR, proline was considered to be the molecule which inhibited the suspension culture growth.

摘要

无 NaCl 的 LS 培养基中的螺旋藻悬浮培养物的生长被 2 mM 脯氨酸抑制了 54%。在含有 260 mM NaCl 的培养基中，10 mM 脯氨酸的抑制生长作用仅为 22%。在 0 或 260 mM NaCl 存在下培养悬浮液时，通过 13 C NMR 和茚三酮分析跟踪 10 mM L-[1-(13) C]脯氨酸的摄取和代谢。在所有培养基中，在 72 小时内，外源性脯氨酸的摄取率为 85%至 92%。在 10 mM 脯氨酸和无 NaCl 的情况下，细胞脯氨酸达到 51.5 μmoles/g FW 的最大值，而在未生长在脯氨酸上的悬浮液中仅为 1.9 μmoles/g FW。在含有 260 mM NaCl 和脯氨酸的培养基中，细胞脯氨酸达到 59-65 μmoles/g FW，而在不含脯氨酸的对照中为 30-40 μmoles/g FW。脯氨酸-C1 中的 13 C 标记要么保留在脯氨酸中，要么通过 TCA 循环的分解代谢消失，大概作为二氧化碳释放。由于通过 NMR 未检测到 13 C-脯氨酸的代谢物，因此认为脯氨酸是抑制悬浮培养物生长的分子。