一维至三维中扩散影响的荧光猝灭动力学。

Diffusion-influenced fluorescence quenching dynamics in one to three dimensions.

机构信息

Department of Physical Chemistry, Uppsala University, Box 532, S-751 21, Uppsala, Sweden.

出版信息

J Fluoresc. 1992 Mar;2(1):7-21. doi: 10.1007/BF00866384.

Abstract

The fluorescence decay curves obtained from diffusion-influenced quenching in various spatial dimensions are discussed. The two-dimensional quenching has, because of intractable fitting functions, previously been dealt with only in the completely diffusion-controlled case (corresponding to the Smoluchowski boundary condition). In this paper, an approximation for the two-dimensional (2D)-quenching behavior with the Collins-Kimball boundary condition is presented. The nonlinear least-squares method has been used to analyze simulated decay data. The consequences the choice of an incorrect model has on the final results as well as the possibility to discriminate between different dimensionalities are investigated. Also, some inherent properties of the fitting functions are studied.

摘要

讨论了在不同空间维度下通过扩散影响猝灭获得的荧光衰减曲线。由于难以拟合的函数，二维猝灭之前仅在完全扩散控制的情况下（对应于 Smoluchowski 边界条件）进行了处理。在本文中，提出了 Collins-Kimball 边界条件下二维（2D）猝灭行为的一个近似。使用非线性最小二乘法分析了模拟的衰减数据。研究了选择不正确模型对最终结果的影响，以及区分不同维度的可能性。此外，还研究了拟合函数的一些固有特性。