蛋白质的银染：与聚酯片结合的二维凝胶的标准化程序。
Silver staining of proteins: standardized procedure for two-dimensional gels bound to polyester sheets.
作者信息
Granier F, de Vienne D
出版信息
Anal Biochem. 1986 May 15;155(1):45-50. doi: 10.1016/0003-2697(86)90222-8.
PMID:2424337
Abstract
A system for the simultaneous silver staining of numerous two-dimensional gels bound to polyester sheets is proposed. Under controlled conditions, a very good reproducibility of staining can be obtained, even between different experiments. Procedures to avoid artifacts due to polyester sheets (background, silver mirror, and vertical streaking) are described.
摘要
提出了一种用于同时对大量附着在聚酯片上的二维凝胶进行银染的系统。在受控条件下，即使在不同实验之间，也能获得非常好的染色重现性。描述了避免因聚酯片产生伪像（背景、银镜和垂直条纹）的方法。
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