Department of Botany, University of Vermont, 05405, Burlington, VT, USA.
Theor Appl Genet. 1985 Dec;71(3):527-31. doi: 10.1007/BF00251200.
Chloroplast DNA (ctDNA) from the tuberbearing Solanum species tuberosum, vernei, phureja, and chacoense has been compared by restriction endonuclease analysis. Digestion by Hind III or Xba I reveal no differences, but digestion with Bam HI and Eco RI reveals minor differences in the ctDNA among these species. The ctDNA restriction patterns of the tetraploid common cultivated potato of North America and Europe, S. tuberosum ssp. tuberosum and the South American tetraploid, S. tuberosum ssp. andigena are identical for all four restriction endonucleases. These data suggest that ssp. tuberosum and ssp. andigena contain similar ctDNA and therefore may share a common ancestor, or direct lineage. The ctDNA restriction patterns of S. vernei and S. chacoense are identical for all four restriction endonucleases, and S. phureja ctDNA, can be distinguished from the other diploid ctDNAs by digestion with Bam HI. None of the diploids analyzed contain ctDNA identical to the tetraploids and therefore either did not contribute their chloroplast genomes to the evolution of the tetraploids, or the ctDNA has diverged since this evolutionary event. The ctDNAs studied did not contain restriction polymorphisms which could be correlated to cytoplasmic male sterility in Solanum. This is the first demonstration of ctDNA diversity in the tuber-bearing Solanum species.
已通过限制内切酶分析比较了产块茎的茄属植物（Solanum）物种（包括马铃薯、委夜茄、匍匐堇菜和智利番茄）的叶绿体 DNA（ctDNA）。用 Hind III 或 Xba I 消化时未发现差异，但用 Bam HI 和 Eco RI 消化时，这些物种的 ctDNA 存在微小差异。北美和欧洲常见的四倍体栽培马铃薯（Solanum tuberosum ssp. tuberosum）和南美洲的四倍体 S. tuberosum ssp. andigena 的 ctDNA 限制图谱在这四种限制内切酶中完全相同。这些数据表明，ssp. tuberosum 和 ssp. andigena 含有相似的 ctDNA，因此可能有共同的祖先或直接的亲缘关系。委夜茄和智利番茄的 ctDNA 限制图谱在这四种限制内切酶中完全相同，而匍匐堇菜的 ctDNA 可以通过 Bam HI 消化与其他二倍体 ctDNA 区分开来。分析的所有二倍体都不含有与四倍体相同的 ctDNA，因此它们要么没有将其叶绿体基因组贡献给四倍体的进化，要么 ctDNA 在这一进化事件后发生了分歧。研究中的 ctDNA 不包含与茄属细胞质雄性不育相关的限制多态性。这是首次在产块茎的茄属植物中证明 ctDNA 的多样性。
