Scotti N, Monti L, Cardi T
CNR-IGV, Institute of Plant Genetics, Research Division Portici, via Università 133, 80055, Portici, Italy.
Theor Appl Genet. 2003 Dec;108(1):87-94. doi: 10.1007/s00122-003-1406-x. Epub 2003 Sep 3.
Nuclear-cytoplasmic interactions can influence fertility and agronomic performance of interspecific hybrids in potato as well as other species. With the aim of assessing the potential value of a novel recombinant cytoplasm derived by interspecific somatic hybridization, backcross progeny were produced by crossing a somatic hybrid between Solanum tuberosum (tbr) and the wild incongruous species S. commersonii (cmm) with various potato clones. BC1 clones were evaluated for male fertility and other agronomic traits. Male fertility clearly depended on the cross direction and the cytoplasm source. Genotypes with cytoplasms sensitive to nuclear genes derived from Solanum commersonii and inducing male sterility showed identical mtDNA composition, as based on mtDNA analyses with various PCR-based and RFLP markers. On the other hand, genotypes with cytoplasms not inducing male sterility in the presence of the cmm nuclear genes showed a different mtDNA organisation. Analysis of cpDNA confirmed similarity of cytoplasmic composition in CMS-inducing genotypes and clear differences with the others. Genotypes with recombinant cytoplasm induced by somatic hybridization generally showed similar agronomic performances in reciprocal hybrids with tbr cytoplasm, suggesting that the novel cytoplasm can be used in potato breeding.
核质互作会影响马铃薯以及其他物种种间杂种的育性和农艺性状表现。为了评估通过种间体细胞杂交获得的新型重组细胞质的潜在价值，将马铃薯（tbr）与野生不亲和物种康氏茄（cmm）之间的体细胞杂种与多个马铃薯无性系杂交，产生了回交后代。对BC1无性系的雄性育性和其他农艺性状进行了评估。雄性育性明显取决于杂交方向和细胞质来源。基于使用多种基于PCR的标记和RFLP标记进行的线粒体DNA分析，对来自康氏茄的核基因敏感并导致雄性不育的细胞质基因型显示出相同的线粒体DNA组成。另一方面，在存在cmm核基因时不导致雄性不育的细胞质基因型显示出不同的线粒体DNA组织。叶绿体DNA分析证实了诱导CMS的基因型中细胞质组成的相似性以及与其他基因型的明显差异。通过体细胞杂交诱导产生重组细胞质的基因型在与tbr细胞质的正反交杂种中通常表现出相似的农艺性状，这表明这种新型细胞质可用于马铃薯育种。
