Department of Agronomy, University of Kentucky, 46546, Lexington, KY, USA.
Theor Appl Genet. 1986 Jun;72(3):328-36. doi: 10.1007/BF00288569.
A population of A. thaliana, produced by self-fertilization of ethylmethane sulfonate treated plants, was exposed to chlorate in the watering solution, and plants showing early susceptibility symptoms were rescued. Among the progeny lines of these plants five were shown to be repeatably chlorate-hypersusceptible. One of these lines (designated C-4) possessed elevated activity of nitrate reductase (NR). The NR activity of mutant C-4 was higher than that of normal plants throughout the life cycle. Nitrite reductase and glutamine synthetase activities of C-4 were normal, as were chlorate uptake rate and tissue nitrate content. The elevated NR activity apparently was responsible for the chlorate hypersusceptibility of C-4. Inheritance studies of NR indicated that the elevated activity of C-4 was probably controlled by a single recessive allele.
用乙基磺酸乙酯处理植物后自花授粉产生的拟南芥种群，在浇水溶液中暴露于氯酸盐中，并拯救了表现出早期敏感性症状的植物。在这些植物的后代品系中，有五条品系被证明是可重复的氯酸盐超敏的。其中一条品系（命名为 C-4）表现出硝酸还原酶（NR）活性升高。在整个生命周期中，突变体 C-4 的 NR 活性都高于正常植物。C-4 的亚硝酸还原酶和谷氨酰胺合成酶活性正常，氯酸盐摄取率和组织硝酸盐含量也正常。显然，NR 活性的升高是 C-4 对氯酸盐超敏的原因。NR 的遗传研究表明，C-4 的高活性可能是由一个隐性等位基因控制的。
