菲可（Ficoll）密度梯度离心法作为一种去除微生物污染和纯化植物原生质体的方法的评估。
An evaluation of Ficoll density gradient centrifugation as a method for eliminating microbial contamination and purifying plant protoplasts.
机构信息
Department of Botany, University of Manchester, M13 9PL, Manchester, UK.
出版信息
Plant Cell Rep. 1986 Aug;5(4):288-91. doi: 10.1007/BF00269824.
PMID:24248249
Abstract
Ficoll density gradient centrifugation was used to generate sterile protoplasts from non-sterile Pteridium cultures. The method was found to drastically reduce levels of fungal and bacterial contamination introduced prior to centrifugation whilst concentrating and purifying protoplasts.
摘要
采用菲克密度梯度离心法从非无菌的蕨类植物培养物中产生无菌原生质体。该方法发现可显著降低离心前引入的真菌和细菌污染水平，同时浓缩和纯化原生质体。
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