植物原生质体的纯化和活力测定。
Purification and viability determinations of plant protoplasts.
机构信息
School of Botany, University of New South Wales, 2033, Kensington, N.S.W., Australia.
出版信息
Planta. 1976 Jan;128(3):213-6. doi: 10.1007/BF00393231.
PMID:24430749
Abstract
A method is described for purifying plant protoplasts from cellular and subcellular debris. The procedure utilizes a density buffer containing 9.6% sodium metrizoate and 5.6% Ficoll. The use of fluorescein diacetate for assessing the viability of plant protoplasts is also reported.
摘要
介绍了一种从细胞和亚细胞碎片中纯化植物原生质体的方法。该方法利用含有 9.6%甲泛影酸钠和 5.6%菲可的密度缓冲液。还报告了使用荧光素二乙酸酯评估植物原生质体活力的方法。
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