Sez. di Torino, CNR, Istituto di Virologia Vegetale, Strada delle Cacce 73, 10135, Torino, Italy.
Arch Virol. 2014 May;159(5):1187-92. doi: 10.1007/s00705-013-1923-0. Epub 2013 Nov 19.
Ourmiaviruses became the object of recent attention for their unusual taxonomic placements among plant viruses. The ourmia melon virus (OuMV) RNA3 encodes a 22-kDa coat protein (CP). Besides its role in virion formation, the OuMV CP facilitates systemic virus spread. In Nicotiana benthamiana, an eGFP-CP fusion protein was localized in the nucleus and preferentially in the nucleolus. By bioinformatics analysis, we identified an arginine- and lysine-rich region at the N-terminus of the CP. Here, we demonstrate by deletion and alanine scanning mutagenesis that this region in the CP is responsible for its preferential accumulation in the nucleolus of host cells.
我们米亚病毒因其在植物病毒中的不同寻常的分类位置而成为近期关注的对象。甜瓜 Ourmia 病毒 (OuMV) 的 RNA3 编码一个 22kDa 的外壳蛋白 (CP)。除了在病毒粒子形成中的作用外，OuMV CP 还促进了系统病毒的传播。在本氏烟中，eGFP-CP 融合蛋白定位于细胞核，并且优先定位于核仁。通过生物信息学分析，我们在 CP 的 N 端鉴定出一个富含精氨酸和赖氨酸的区域。在这里，我们通过缺失和丙氨酸扫描诱变证明，CP 中的这个区域负责其在宿主细胞的核仁中的优先积累。
