Laboratoire de Biologie et Physiologie Végétales, Université de Bordeaux I, Allée des Facultés, F-33405, Talence Cédex, France.
Plant Cell Rep. 1986 Oct;5(5):368-71. doi: 10.1007/BF00268604.
The initial and induced in vivo Nitrate Reductase Activity, the nitrate accumulation by in vitro-produced axillary shoots and plantlets of Pinus pinaster were compared respectively with those of shoots collected from seedlings and whole plants.The usefulness of the nitrate of the medium used for in vitro axillary shoot formation is demonstrated by the occurrence of initial NR activity in the explants. When fed in a non in vitro situation with a 50 mM KNO3 solution, they have the same induced capacity to reduce nitrate as do shoots from seedlings, even though the latter accumulate less nitrate. Plants regenerated in vitro exhibit an ability to reduce nitrate similar to that of seedlings. In both types of plants, the Nitrate Reductase potential is greater in roots than in shoots.
分别比较了栓皮松腋芽和组培苗的初始和诱导体内硝酸还原酶活性、体外产生的腋芽和组培苗的硝酸盐积累量，与从幼苗和整株植物采集的芽进行了比较。培养过程中使用的硝酸盐培养基的有效性可以通过外植体中初始 NR 活性的出现来证明。当用 50mM KNO3 溶液在非体外条件下喂养时，它们与来自幼苗的芽具有相同的诱导硝酸盐还原能力，尽管后者积累的硝酸盐较少。体外再生的植物表现出与幼苗相似的硝酸盐还原能力。在这两种植物中，根中的硝酸还原酶潜力大于芽。
