Lin H S, De Jeu M J, Jacobsen E
The Graduate School Experimental Plant Sciences, Department of Plant Breeding, Wageningen Agricultural University (WAU), PO Box 386, 6700 AJ Wageningen, The Netherlands, , , , , , NL.
Plant Cell Rep. 1997 Sep;16(11):770-774. doi: 10.1007/s002990050317.
A two-step protocol for the induction of shoots from Alstroemeria leaf explants has been developed. Leaf explants with stem node tissue attached were incubated on shoot induction medium for 10 days, and then transferred to regeneration medium. Shoots from the area adjacent to the region between the leaf base and node tissue regenerated within 3 weeks after transfer to the regeneration medium, without a callus phase. The best induction was obtained with Murashige and Skoog medium containing 10 µM thidiazuron and 0.5 µM indole butyric acid. The regeneration medium contained 2.2 µM 6-benzylaminopurine. After several subcultures of the leaf explants with induced shoots, normal plantlets with rhizome were formed. In Alstroemeria, the percentage of responding leaf explants is more important than the number of shoots regenerated per leaf explant, because rhizome formation is the most important factor for micropropagation. The effect of other compounds in the induction medium, including glucose, sucrose, silver nitrate, and ancymidol, on regeneration was also investigated.
已开发出一种从六出花叶片外植体诱导芽的两步方案。带有茎节组织的叶片外植体在芽诱导培养基上培养10天，然后转移到再生培养基上。转移到再生培养基后3周内，从叶基部和节组织之间区域相邻处的芽再生，无愈伤组织阶段。在含有10 µM噻苯隆和0.5 µM吲哚丁酸的Murashige和Skoog培养基上诱导效果最佳。再生培养基含有2.2 µM 6-苄基腺嘌呤。对诱导出芽的叶片外植体进行几次继代培养后，形成了带有根茎的正常植株。在六出花中，有反应的叶片外植体的百分比比每个叶片外植体再生的芽数更重要，因为根茎形成是微繁殖的最重要因素。还研究了诱导培养基中的其他化合物，包括葡萄糖、蔗糖、硝酸银和嘧啶醇对再生的影响。
