在高蔗糖含量的培养基上，通过杂种向日葵（Helianthus annuus L.）未成熟胚胎的直接体细胞胚胎发生和植株再生。

Direct somatic embryogenesis and plant regeneration from immature embryos of hybrid sunflower (Helianthus annuus L.) on a high sucrose-containing medium.

机构信息

Department of Agronomy, Ohio Agricultural Research and Development Center, The Ohio State University, 44691, Wooster, OH, USA.

出版信息

Plant Cell Rep. 1987 Oct;6(5):372-4. doi: 10.1007/BF00269564.

DOI:10.1007/BF00269564

PMID:24248849

Abstract

Somatic embryos of sunflower (Helianthus annuus) were obtained by placing immature zygotic embryos on a high sucrose (12%) containing medium. The somatic embryos were first observed 6 days after culture and a callus intermediate was not formed. Histological examination revealed the classical stages of embryo development. The somatic embryos proliferated directly from the surface of the zygotic embryos and germinated after placement on a low sucrose medium. Seeds have been obtained from regenerated plants.

摘要

向日葵（Helianthus annuus）的体细胞胚是通过将未成熟的合子胚置于高浓度蔗糖（12%）培养基上获得的。培养 6 天后首次观察到体细胞胚，且未形成愈伤组织中间层。组织学检查显示出胚胎发育的经典阶段。体细胞胚直接从合子胚的表面增殖，并在低浓度蔗糖培养基上放置后发芽。已经从再生植株中获得了种子。