Institute of Genetics, Bulgarian Academy of Sciences, 113, Sofia, Bulgaria.
Plant Cell Rep. 1986 Aug;5(4):256-8. doi: 10.1007/BF00269815.
Protoplasts were isolated from seedling roots, hypocotyls, and cotyledons of four cultivars of Helianthus annuus and from leaves of axenic shoot cultures of the wild species H. praecox, H. scaberimus and H. rigidus. Optimal culture conditions were established for the respective protoplast systems, using the agarose bead method of culture. Protoplast division was induced for all the species examined. In the case of the cultivars of H. annuus, hypocotyl and cotyledon protoplast division was sustained leading to callus formation, which in turn, could be induced to produce roots and organised meristematic regions in the presence of NAA and 6-BAP.
原生质体分别从四个向日葵品种的幼苗根、下胚轴和子叶，以及野生向日葵种糙叶向日葵、多毛向日葵和硬向日葵的无菌芽培养物的叶片中分离出来。采用琼脂珠培养法，为各原生质体系统建立了最佳的培养条件。所有受检物种的原生质体都能诱导分裂。对于向日葵品种而言，下胚轴和子叶原生质体的分裂得到维持，导致愈伤组织的形成，而在 NAA 和 6-BAP 的存在下，这种愈伤组织可以进一步诱导生根和形成有组织的分生组织区域。
