Laboratoire de Physiologie végétale moléculaire (UA CNRS 1128), bâtiment 430, Université Paris-Sud, 91405, Orsay, Cedex, France.
Plant Cell Rep. 1988 Oct;7(6):437-40. doi: 10.1007/BF00269532.
Sunflower hypocotyl protoplasts have been isolated and cultured. Optimum plating density for cell division and colony formation was in the range of 5 to 7×10(4) cells/mi in an agarose medium supplemented with BAP (1 mg/l) and NAA (1 mg/l). Plating efficiency was 60% after 21 days of culture. In the resultant culture a mixed population of calli and embryoids was observed. Thirty seven percent of the cell clusters exhibited a developmental pattern similar to an embryoid. Many stages of embryogenesis were observed in the same cultures.
向日葵下胚轴原生质体已被分离和培养。在琼脂培养基中添加 BAP（1mg/L）和 NAA（1mg/L），细胞分裂和集落形成的最佳接种密度范围为 5 到 7×10(4)个细胞/毫升。培养 21 天后，接种效率为 60%。在所得的培养物中观察到一个愈伤组织和胚状体的混合群体。37%的细胞团表现出类似于胚状体的发育模式。在同一培养物中观察到许多胚胎发生阶段。
