从棉花（Gossypium hirsutum L.）原生质体中进行细胞再生和持续分裂。
Cell regeneration and sustained division of protoplasts from cotton (Gossypium hirsutum L.).
机构信息
University of Arizona, Forbes Building No. 36, Room 201, 85721, Tucson, AZ, USA.
出版信息
Plant Cell Rep. 1987 Dec;6(6):470-2. doi: 10.1007/BF00272785.
Protoplasts were isolated from 12 day old subcultured phytohormone habituated callus tissue of Gossypium hirsutum L. (0.5% cellulysin-Calbiochem, 0.6% macerase-Calbiochem, 0.7M mannitol, and pH 5.0). After separation and purification (0.35M sucrose floatation medium), the protoplasts were cultured (K3 media of Kao et al., 1974 with 0.9 μM BAP, 5 μM IAA and 0.35M sucrose) in both liquid and solid medium at a density of 5×10(5) protoplasts/ml. Four weeks after isolation, cell regeneration and callus formation was observed.
原生质体从培养了 12 天的、经过植物激素驯化的陆地棉（Gossypium hirsutum L.）愈伤组织中分离得到（0.5%纤维素酶-Calbiochem，0.6%离析酶-Calbiochem，0.7M 甘露醇，pH5.0）。分离和纯化后（0.35M 蔗糖漂浮培养基），在液体和固体培养基中以 5×10(5)个原生质体/ml 的密度培养原生质体（Kao 等人的 K3 培养基，0.9μM BAP、5μM IAA 和 0.35M 蔗糖）。分离 4 周后，观察到细胞再生和愈伤组织形成。
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