University of Arizona, Forbes Building No. 36, Room 201, 85721, Tucson, AZ, USA.
Plant Cell Rep. 1987 Dec;6(6):470-2. doi: 10.1007/BF00272785.
Protoplasts were isolated from 12 day old subcultured phytohormone habituated callus tissue of Gossypium hirsutum L. (0.5% cellulysin-Calbiochem, 0.6% macerase-Calbiochem, 0.7M mannitol, and pH 5.0). After separation and purification (0.35M sucrose floatation medium), the protoplasts were cultured (K3 media of Kao et al., 1974 with 0.9 μM BAP, 5 μM IAA and 0.35M sucrose) in both liquid and solid medium at a density of 5×10(5) protoplasts/ml. Four weeks after isolation, cell regeneration and callus formation was observed.
原生质体从培养了 12 天的、经过植物激素驯化的陆地棉（Gossypium hirsutum L.）愈伤组织中分离得到（0.5%纤维素酶-Calbiochem，0.6%离析酶-Calbiochem，0.7M 甘露醇，pH5.0）。分离和纯化后（0.35M 蔗糖漂浮培养基），在液体和固体培养基中以 5×10(5)个原生质体/ml 的密度培养原生质体（Kao 等人的 K3 培养基，0.9μM BAP、5μM IAA 和 0.35M 蔗糖）。分离 4 周后，观察到细胞再生和愈伤组织形成。
